Cisplatin a549 ic50

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August undefined, 2024

WebMar 27, 2024 · Cisplatin sensitivity (IC50) of the 9 NSCLC cell lines was determined using in vitro cytotoxicity assay (CCK-8 assay, Figure 1 (b) ). Based on the cut-off IC50 cisplatin concentration of 4 μ M, the cell lines were classified into cisplatin sensitive (IC50 ≤ 4 μ M) and resistant (> 4 μ M) groups. WebThis study was designed to define whether exposure to cisplatin could affect the sensitivity to EGFR TKIs because conflicting results have been presented. We established sublines that are resistant to cisplatin from EGFR wild-type cells (A549 and H460) and EGFR mutant cells (PC-9 and HCC827).

Cisplatin-resistant A549 non-small cell lung cancer cells can be ...

WebIC 50 values (mM) of doxorubicin (DOX) alone and in combination with L-canavanine (L-CAV) (constant ratio). Source publication L-Canavanine potentiates the cytotoxicity of doxorubicin and... WebThe median inhibitory concentration (IC50 ) in cisplatin-treated A549 and A549/DDP cells was 5.769 ± 0.24 μmol/L and 28.373 ± 0.96 μmol/L, respectively; the cisplatin resistance of A549 cells was about five times that of A549/DDP cells. dura automotive systems india

Cisplatin resistance-related multi-omics differences and the ...

WebJul 26, 2024 · In A549 cells, cisplatin exposure led to a significantly higher expression of genes coding for proteins mediating G2/M arrest and apoptosis (mouse double minute 2 … WebFeb 28, 2024 · All of these new complexes have been tested on three human cancer cell lines (A549 lung, SKOV3 ovarian, and MCF-7 breast cancer cell lines) and studied for … WebImmunofluorescence results elucidated that cellular ROS level in Cisplatin-induced A549 cells increases in a dose-dependent manner. Both immunofluorescence and flow cytometry results revealed that the apoptotic rate of A549 cells increases with the elevation of Cisplatin dose. cryptloid

Cisplatin resistance in non-small cell lung cancer cells is …

Determination of IC50 values of anticancer drugs on cells by D2O ...

WebApr 13, 2024 · Cisplatin‑resistant A549 (A549/CDDP) and H460 (H460/CDDP) cells were first established and were then transfected with negative control small interfering (si)RNA (si‑NC) or TRPC1 siRNA (si‑TRPC1). ... TRPC1 silencing decreased the IC50 value of CDDP compared with the si‑NC group in A549/CDDP (11.78 vs. 21.58 µM; P<0.01) and … WebThe IC 50 of cisplatin on A549 cells was 4.07±0.32, 2.26±0.43, and 1.52±0.41 µg/mL, for 24, 48, and 72 hours, respectively. Thus, we used 4, 2, and 1 µg/mL of cisplatin to treat A549 cells... crypt lockerWebJan 17, 2024 · A simple, sensitive and repeatable D 2 O-single cell Raman spectroscopy method is developed to quantify the inhibitory activity of anticancer drugs on cancer cell metabolism. The IC 50 values obtained from A549 cells incubated with cisplatin and taxol are comparable with results of CCK-8 and ATP luminescent cell viability assays. duraar cricket

"WebThe IC50 value of cisplatin was also assessed with CCK-8 assays. The western blotting for AKT, p-AKT, S6K and p-S6K were performed in cells with TAZ knockdown or overexpression. When the AKT/mTOR pathway was blocked in A549 and H460 with TAZ overexpression, cisplatin sensitivity was assessed by IC50 value. " - Cisplatin a549 ic50

Cisplatin a549 ic50

Enhanced expression of lncRNA ZXF1 promotes cisplatin …

WebAug 1, 2024 · A549 and A549/TAX cells were seeded in 100 mm cell plate, after culturing for 24 h, CHIP assay was conducted. CHIP was performed with rabbit smad3 antibody according to the manufacturer’s protocol (Millipore, USA). Then use the TIANGEN kit to extract DNA according to the instructions. PCR was used to test the performance of the … WebThe cisplatin sensitivity of A549/DDP by the MTT assay, IC50 = 2.2μg/mL. The cisplatin sensitivity of A549 by the MTT assay, IC50 = 0.8μg/mL (B) Wound healing assays were …

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WebNational Center for Biotechnology Information WebMar 22, 2024 · a A549 and A549/DDP cells were exposed to different concentrations of cisplatin (1, 10, 20, 40, 80, 160 μM) for 48 h, followed by the determination of cell …

WebApr 11, 2024 · Obviously, the resistance to cisplatin varies among different cancers. In the Depmap database, we successfully classify different tumor cell lines according to their source of cancer, so as to obtain the average IC50 value of cisplatin of related cancers.

WebIC50 values of cisplatin were determined for A549/SEN and A549/CR cells using inhibition dose-response curves with variable slopes. For the colony formation assay, 500 cells were seeded in 12-well plates and cultured in … WebNov 19, 2013 · A549 cells were exposed to cisplatin at an IC50 dose (12 µM) for 24 h; then, cells were co-stained with Annexin V and MitoSox and analyzed by flow cytometry. (A-C) Annexin V-negative subpopulation (about 15-20%) was gated in each sample in order to exclude the apoptotic fraction of cells from the analysis.

WebJul 23, 2015 · The IC50 of cisplatin for A549 and SPC-A1 cells under hypoxia was 3.38-fold and 1.57-fold higher as compared to that under normoxia respectively as showing by the …

WebMar 22, 2024 · We developed CDDP-resistant A549 (A549/DDP) cells through prolonged incubation with the drug and performed RNA-seq on RNA extracts to determine … durabar thailandWebFeb 28, 2024 · Complexes 36–40 were tested against lung carcinomas A549 and A549/DDP, cisplatin-insensitive cells, and normal healthy (HL-7702) cells. ... (IC50 = 3.87 μM), complex 40 (IC 50 = 0.91 μM), and cisplatin (IC 50 = 50.12 μM), all of which showed lower cytotoxic effects against HL-7702 compared with the standard approved regimen … dura automotive systems muscle shoalsWebCisplatin (NSC 119875, Cisplatinum, cis-diamminedichloroplatinum II, CDDP, cis DDP, DDP) is an inorganic platinum complex, which is able to inhibit DNA synthesis by conforming DNA adducts in tumor cells. Cisplatin activates ferroptosis and induces autophagy. Solutions are best fresh-prepared. durabar speeds and feedsWebJan 17, 2024 · A simple, sensitive and repeatable D2O-single cell Raman spectroscopy method is developed to quantify the inhibitory activity of anticancer drugs on cancer cell … dura backflow valve test kitWebNov 29, 2024 · This study revealed that cisplatin resistant A549 lung cancer cells can be identified by their increased levels of mitochondrial mass. However, Mito-High cells … crypt logoWebThe treatment of A549 cells with CLEFMA improved cisplatin efficacy and decreased cisplatin ototoxicity by regulating apoptosis-inducing factor and caspase-12 [12]. CLEFMA elicits apoptotic cell ... durabase mats specsWebDDP with IC50 of 133.3 and 63.7 μM was obtained for A549/DDP cells with siNC or siUSP14#1 transfection, respectively (Figure 2A). DDP with IC50 of 57.7 and 86.2 μM was obtained for A549 cells with empty vector or USP14 expression vector transduction, respectively (Figure 2B). crypt locker flash drive protection