Orc2 antibody drosophila
WebNov 2, 2011 · We thank I. Chesnokov and the Bloomington Drosophila Stock Center (Bloomington, IN) for flies, S. Bell and M. Botchan for α-Orc2 antibodies, J. Powers of the Indiana Light Microscopy Imaging Center (Bloomington, IN) for helpful advice, and M. Lilly and members of the Calvi lab for advice and comments on the manuscript. We thank M. … WebNational Center for Biotechnology Information
Orc2 antibody drosophila
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WebApr 13, 2024 · This study shows that Drosophila germ granules, which initially protect RNAs, subsequently become sites of selective mRNA degradation; this plasticity supports germ cell development. ... 50 μg/mL tRNA), embryos were incubated with primary antibody in PBHT overnight at 4°C with rocking. Primary antibodies used: 1:250 rabbit anti-CCR4 , 1:250 ... WebThe origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast …
WebJan 12, 2007 · We find that the developmental timing, epigenetic regulation, and Orc2 protein binding have been strictly conserved over 40 million years of evolution. We also present evidence that additional loci may be amplified in some species. WebAug 30, 2011 · ORC function is required to allow both stages of replication initiation at DAFC-34B. DNA copy number was examined in a female-sterile allele of ORC, orc2fs293, which reduces gene amplification. orc2k43 is a null allele. Amplification in the sibling heterozygotes shows typical amplification levels.
WebTo verify the phosphorylation at Thr-116 and Thr-226 of ORC2, we generated phosphoantibodies that specifically detected phospho-Thr-116 or phospho-Thr-226 of ORC2. The α-pT116 and α-pT226 antibodies recognized GST-ORC2 expressed in 293T cells (Fig. 1C). However, neither phosphoantibody detected mutant forms of the protein with Thr to … WebRabbit anti-Drosophila melanogaster (Fruit fly) Orc2 Polyclonal antibody: Uniprot No. Q24168: Target Names: Orc2: Alternative Names: Orc2 antibody; CG3041 antibody; Origin …
WebMay 1, 2004 · Antibody to the origin recognition complex subunit 2 (ORC2)recognizes large foci that localize to the endogenous chorion gene loci and to active transgenic constructs …
WebThe origin-recognition complex (ORC) has an essential role in defining DNA replication origins [1] and in chromosome segregation [2, 3]. Recent studies in Drosophila orc2 mutants [4–6], and in human cells depleted of ORC2 [3], have suggested that this factor is also implicated in mitotic chromosome assembly. We asked whether ORC was required for M … philips alpha wifiWebDrosophila Myb recognizes a highly conserved DNA sequence10,11, but the specific binding properties of the glycerol gradient fractions might be more complex than that of … philip salstrom mdWebPhosphospecific antibodies were generated using synthetic peptides corresponding to amino acids 112–120 (CELAKpTPQKS) and 221–230 (CPVGKEpTPSKR) of ORC2 for anti … philips alpha缺點WebJun 23, 2024 · The process is likely conserved since the human ORC2 WHD interacts with ORC1 in the ORC2-5 structure and occupies a number of dynamic positions 58. The Cdc6 WHD undergoes a series of movements ... philips alpha pttWebDec 14, 2000 · This pattern of staining was also observed using affinity-purified ORC2 antibody and in three Drosophila cell lines following either paraformaldehyde or methanol fixation (Fig. 2 b). ORC2 staining is remarkably similar to that of Xenopus ORC1, which is also absent from metaphase chromosomes but present on anaphase chromatids [33]. It … philips altexWebOct 2, 2001 · Immunostaining of the Drosophila embryos was performed as described by using affinity-purified antibody raised against Drosophila ORC2 and ORC6 proteins. Results Six different mutant complexes and wild-type recombinant ORC were prepared as … trustom pond hikingWebOct 8, 2010 · IP with Orc2 antibody followed by immunoblot with ORCA polyclonal antibody (pAb) showed the association of Orc2 with endogenous ORCA (molecular weight of ORCA ∼70kDa; Figure 1 D). Finally, immunoprecipitation with ORCA pAb in human cells clearly demonstrated the association of ORCA with ORC subunits ( Figure 1 E). trustone account number